To investigate in detail the effect of physiological temperatures on CFTR-dependent currents in airway epithelial cells in the context of a pharmacological correction of CFTR membrane expression and function, we used the CF bronchial epithelial CFBE41o− cell line expressing either F508del- or WT-CFTR (CFBE-F508del or CFBE-WT respectively). The gene discussed is CFTR; the disease is cystic fibrosis.