For the other CpG sites there was evidence for a difference in DNA methylation between tumour and healthy adjacent tissue at several sites in both adenocarcinoma and squamous cell carcinoma, with consistent differences for CpG sites in ALPPL2 (cg2156642, cg05951221 and cg01940273), as well as cg23771366-PRSS23, cg26963277-KCNQ1, cg09935388-GFI1, cg0101332-ARRB1, cg08709672-AVPR1B and cg25305703-CASC21. This evidence concerns the gene PRSS23 and adenocarcinoma.