For cg05575921-AHRR, there was no strong evidence for differential methylation between adenocarcinoma tissue and adjacent healthy tissue (P = 0.963), and weak evidence for hypermethylation in squamous cell carcinoma tissue (P = 0.035) (Figure 4; Supplementary Table 10, available as Supplementary data at IJE online). This evidence concerns the gene AHRR and squamous cell carcinoma.