In conclusion, active PLK1 phosphorylated at T210, abundant in TGF-β-induced metastatic NSCLC, promotes metastasis through the upregulation of genes related to the TGF-β-related signaling pathway including TNFAIP6, which amplifies NSCLC’s metastatic properties by forming a positive loop. Here, TGFB1 is linked to non-small cell lung carcinoma.