We measured the protein expression levels of Hippo pathway proteins (Rassf-1, Mst1, Mst2, Sav1, Mob1, p-Mob1, YAP, p-YAP, and CTGF) and metastasis proteins (E-cadherin, MMP-9, Twist, and Vimentin) in the control and UA-treated xenograft tumor tissues to further evaluate whether UA regulates the tumorigenesis of gastric cancer in vivo through activating the Hippo pathway. The gene discussed is TWIST1; the disease is gastric cancer.