Therefore, to better understand the effect of GH on MITF expression, we treated human melanoma cells with increasing doses (0, 50, 100, 200 ng/mL) in presence or absence of drug and followed the expression of MITF and MITF target genes across three time points (6, 24, and 48 h) in both the melanotic melanoma cell SK-MEL-30 and the amelanotic cell SK-MEL-28. The gene discussed is GH1; the disease is melanoma.