GJA1 and melanoma: In contrast to MB49 cells, which did not induce Cx43 polarization to the contact site with pMEL-1 CTLs, B16F10 melanoma cells did acquire calcein from pMEL-1 CTLs after 30 min of co-culture (Figure 2A), concomitant with the Cx43 polarization to the cell-to-cell contact site.