To this end, we used CTLs isolated from pMEL-1 mice, which specifically recognize the epitope XXXRNQDWL for the MAA gp100 endogenously expressed by the syngeneic B16F10 melanoma cells, and a human CTL clone specific for the HLA-A2-restricted MART-127–35 antigen, an MAA expressed in the HLA-A2+ Mel1 melanoma cell line. The gene discussed is PMEL; the disease is melanoma.