We observed a structure resembling actin rings in pMEL-1 CD8+ CTLs upon antigen recognition of target B16F10 melanoma cells (Figure 1) and, only upon this antigen-specific driven process, Cx43 polarizes to the cell-to-cell contact sites, indicating that Cx43 accumulates in the cytotoxic immunological synapses of CTLs. This evidence concerns the gene GJA1 and melanoma.