ZNF177 and colorectal carcinoma: To further confirm the abnormal methylation phenotype, we examined the methylation status of LMX1A, SOX1, and ZNF177 in five CRC cell lines (HCT8, HCT116, HT29, SW480, and SW620) using the MSP assay (Figure 1B) and quantified the methylation levels using the Q-MSP assay (Figure 1C).