SOX1 and colorectal carcinoma: Furthermore, we performed a receiver operating characteristic (ROC) curve analysis to discriminate between the CRC tissue samples and their nontumor counterparts (supporting information, Figure S1) and determined that the methylation frequencies of LMX1A, SOX1, and ZNF177 were 22.5%, 13.9%, and 12.6%, respectively, under the best cut-off values (Table 1).