Interestingly, the hypermethylation levels of the four candidates were considerably increased in HCC subgroups associated with HBV infection (with and without cirrhosis) compared to HCC subgroups associated with HCV infection (with and without cirrhosis), suggesting a high diagnostic value of the four candidates APC, COX2, RASSF1A, and miR-203 in HCC associated with HBV infection. The gene discussed is RASSF1; the disease is hepatocellular carcinoma.