AIF1 and ischemia: In the HFD 2-min TI group, Iba-1+ microglia were more hypertrophied after ischemia (Figure 6A-a5,6A-a8): at 5 days post-ischemia, strongly immunostained and hypertrophied Iba-1+ microglia were concentrated in the stratum pyramidale where the death of the CA1 pyramidal cells was discovered, and the ROD of Iba-1+ structures was very high (about 198% of the HFD sham group, p < 0.001) (Figure 6A-a8,B).