In the HFD/RAPA 2-min TI group, IL-1β and TNF-α levels were significantly decreased (about 1.7-fold, p < 0.001, and 1.8-fold, p < 0.001 of the HFD 2-min TI group, respectively) at 2 days post-ischemia, and the decreased levels were not altered until 5 days post-ischemia. Here, TNF is linked to ischemia.