In the HFD 2-min TI group, IL-1β and TNF-α immunoreactivity was further significantly increased (about 167%, p < 0.001, and 133%, p < 0.001 of the HFD sham group, respectively) at 2 days post-ischemia (Figure 7A-a5,7B-b5,C,D), and each immunoreactivity was rarely detected in CA1 pyramidal cells at 5 days post-ischemia due to death of CA1 pyramidal cells following 2-min TI (Figure 7A-a8,7B-b8,C,D). This evidence concerns the gene TNF and ischemia.