TNF and ischemia: In addition, in the HFD/RAPA 2-min TI group, IL-1β and TNF-α immunoreactivity was significantly lower (about 63%, p < 0.001 and 71%, p < 0.001, respectively) than the HFD 2-min TI group at 2 days post-ischemia, and the immunoreactivity sustained until 5 days post-ischemia (Figure 7A-a6,7A-a9,7B-b6,7B-b9,C,D).