In the HFD/RAPA 2-min TI group, the ROD of Iba-1+ structures at 2 and 5 days post-ischemia was significantly low (about 81%, p < 0.05 and 66%, p < 0.001 of the HFD 2-min TI group, respectively) compared to the HFD 2-min TI group (Figure 6A-a6,6A-a9,B). This evidence concerns the gene AIF1 and ischemia.