In the HFD/RAPA 2-min TI group, no changes in NeuN+ or F-J B+ CA1 pyramidal cells were found at 2 days post-ischemia (Figure 4A-a6,4A-a6′,B,C), and, at 5 days post-ischemia, NeuN+ CA1 pyramidal cells were significantly increased (71 cells/250 × 250 μm, p < 0.001) and F-J B+ CA1 pyramidal cells were significantly decreased (9 cells/250 × 250 μm, p < 0.001) compared to those in the HFD 2-min TI group (Figure 4A-a9,4A-a9′,B,C). The gene discussed is RBFOX3; the disease is ischemia.