In the HFD/RAPA 2-min TI group, mTOR and p-mTOR immunoreactivity at 2 days post-ischemia was significantly lower (about 69%, p < 0.001, and 77%, p < 0.001 of the HFD 2-min TI group, respectively) than the HFD 2-min TI group, and, their immunoreactivity was maintained until 5 days post-ischemia (Figure 8A-a6,8A-a9,8B-b6,8B-b9,C,D). Here, MTOR is linked to ischemia.