Additionally, the autophagy inducer rapamycin (rapa) (Fulda and Kogel, 2015; Marino et al., 2014) induced accumulation of LC3B in colorectal carcinoma cells, albeit to a somewhat lower degree than NVP and NVP‐Iso, and expectedly reduced constitutive activity of the mammalian target of rapamycin that was not altered by NVP and NVP‐Iso (Fig. S8A). This evidence concerns the gene TRERF1 and colorectal carcinoma.