CD4 and ulcer disease: In another study in Colombia, two groups were described: first the people without any ulcer but their Leishmanin skin test were positive, the other one, infected by L. panamensis the amount of CD4+ CD25+ CD127lowTregs were determined in peripheral blood and IL-10, INF-γ and Foxp3 expression were evaluated in skin biopsies and the result indicated that the second group had more Tregs in their blood and lesions (28).