RHOA and congestive heart failure: To further investigate the effect of miR-93 targeting LIMK1 gene on the expression of miR-93, LIMK1, RhoA and ROCK1 in myocardial tissues of rats with CHF, RT-qPCR and western blot assay were involved and the results showed that in contrast to the miR-93 mimics + OE-NC group, no obvious change was found in the expression of miR-93 in the miR-93 mimics + OE-LIMK1 group (P > 0.05), and the expression levels of LIMK1, RhoA and ROCK1 were obviously increased (all P < 0.05; Figure 1A–1C).