JAK2 and myelodysplastic syndrome: In the 16 MPN, the median VAF of the main variant was 0.31 (range 0.09–0.5), and the main variant affected the signal transduction gene group (38%, 6/16), particularly JAK2. Finally, for the ten MDS/MPN, the median VAF of the main variant was 0.41 (range 0.4–0.5).