Correspondingly, we found that using an intracolonic itch cocktail of CCDC, CQ, and BAM8-22 to concurrently activate TGR5, MrgprA3, and MrgprC11 on colonic afferents in CVH mice caused decreases in locomotion and increased grooming and thigmotaxis, indicative of anxiety-like behavior. This evidence concerns the gene GPBAR1 and Anxiety.