It has been reported that silencing of CHI3L1 using siRNA in cells has shown that a decrease in CHI3L1 levels in U87MG cells can significantly reduce cell viability.29 In this study, two colon cancer cells were successfully infected with lentivirus, which could overexpress CHI3L1 stably, and laid a foundation for downstream biological function research. Here, CHI3L1 is linked to colonic neoplasm.