We performed acetylcholinesterase (AchE) assays, NanoSight analysis and Western blot analysis to characterize EVs released from MCF-7 and MDA-MB-231 human breast cancer cells and K562 leukemia cells treated for five days at 37° C with the IC50 for various peptides, or 1 μM MKT-077, 200 μM omeprazole and 300 μM DMA. The gene discussed is ACHE; the disease is breast cancer.