The PCR results confirmed the RNAseq analysis for TNFRSF19, Eef1a2, Kit, Top2a, Sod3, and Cxcl12. Although tumor samples treated with empty nanoparticles were not included in the original RNAseq comparison, they were analyzed using real-time PCR to evaluate the expression of a few genes that were significantly regulated by NanoTLZ. The gene discussed is EEF1A2; the disease is neoplasm.