This was particularly true in animal experiment that anti-bone metastatic role of silencing ZEB1 in PCa cells was significantly reversed by injection of antagomir-33a-5p, as indicated by an increased bone metastatic sites, osteolytic area and bone burden, and reduced bone metastasis free survival (Figure 5K-O and Figure S5A-C). This evidence concerns the gene ZEB1 and posterior cortical atrophy.