Significantly elevated serum concentrations of IFN-γ and IL-17 (p<0.05; Figure 11E) and remarkably higher presence of IFN-γ and IL-17 producing neutrophils (Figure 11F-G, p<0.05) and CD4+ T cells (Figure 11H-I, p<0.01 for Th1 and p<0.05 for Th17 cells) were observed in the kidneys of CY+CDDP- and anti-CD25+CDDP-treated WTWTDCsPam3CSK4 and Gal-3-/-WTDCsPam3CSK4mice, indicating that depletion of Tregs diminished Gal-3-dependent capacity of TLR-2-primed DCs to suppress IFN-γ and IL-17 driven inflammation in AKI. Here, TLR2 is linked to acute kidney injury.