To explore the biological function of TCF12 in HCC, endogenous TCF12 expression levels in a set of hepatoma cell lines and L02 cells were initially measured by RT-qPCR and western blot; the data revealed that TCF12 was strongly expressed in Hep3B and Huh7 cells; moderately expressed in MHCC- LM3, MHCC-97H, PLC/PRF/5, SK-Hep 1 and Li7 cells; and weakly expressed in MHCC-97L, SMMC- 7721 and L02 cells (Figure S1A). The gene discussed is TCF12; the disease is hepatocellular carcinoma.