CALR and malignant colon neoplasm: Therefore to evaluate whether the EV-formulations could induce an immunogenic cell-death program, the expression of specific markers, such as exposure of calreticulin on the cell surface and extracellular release of ATP 46, was measured on murine lung and colon cancer cells respectively treated with 10 particles/cell EV-LL/2 and EV-MC-38, Virus, EV-Virus-LL/2, EV-Virus-MC-38.