After S1PR1 expression was significantly downregulated in HCT116, the proliferation (Fig. 2b), migration (Fig. 2c), and invasion (Fig. 2d) capabilities were significantly decreased in vitro when compared with the control group and resulted in a significant decrease in subcutaneous tumor size (Fig. 2e) and fewer liver metastases in vivo (Fig. 2f). The gene discussed is S1PR1; the disease is neoplasm.