TNF and pachyonychia congenita: Meanwhile, the KEGG pathway analysis also exhibited the top 20 most enrichment pathways, including central carbon metabolism in cancer, TNF signaling pathway, Focal adhesion, MAPK signaling pathway, PI3K-Akt signaling pathway, HIF-1 signaling pathway, Pyrimidine metabolism, Purine metabolism and so on (Fig. 5b), which indicated that these pathways might be involved in the nigericin-mediated anti-cancer effects in PC cells.