Thirty-nine target genes were identified as down-modulated by miR-223 in RMS, among which were FOXO1, FOXO3, PAX3, and PAX4. Three genes resulted in the most anti-correlated: CDS1, SP3, and ARTN. In RMS tumor tissues, integration analysis showed an up-regulation of EMT pathways correlated with miR-223 levels speculating that regulation of tumor suppressor Fbxw7 could be responsible for this pro-tumorigenic effect (Figure 4A and Supplementary Figure S2A). This evidence concerns the gene PAX3 and neoplasm.