To corroborate these findings, both amiloride (p<0.0001) and S18 (p=0003) inhibited ASC-speck formation in CF monocytes (Figure 5D), and finally, inhibition of amiloride-sensitive channels did not modulate TNF levels (Figure 5—figure supplement 1F), suggestive of a specific ENaC-NLRP3 axis. Here, NLRP3 is linked to cystic fibrosis.