The exclusion criteria were hypersensitivity to tetracycline or minocycline medicine, Pregnant and breast feeding women, history of systemic lupus erythematosus (SLE), history of receiving chronic steroid treatment and isotretinoin, Pre-existing hepatic (AST, ALT greater than 3 times the upper limit of normal) or renal failure (BUN/ Creatinine 20:1; creatinine > 2 mg/dL), Significant leucopoenia (white blood cell count less than 0.5 times the lower limit of normal), Thrombocytopenia (platelets < 75,000/mm3), incidence of pseudomembranous colitis and Patients who expired 48 hours after injury. This evidence concerns the gene GPT and systemic lupus erythematosus.