The results in this study showed that GBEE (50, 100, and 200 mg/kg) had no significant effect on the expression of PI3K and Akt protein in B16 transplanted tumor, but the protein level of p-PI3K and p-Akt was down-regulated in a dose-dependent manner, indicating that the role of GBEE in the intervention of HIF-lα/VEGF pathway may be related to the regulation of PI3K/Akt signaling pathway. Here, VEGFA is linked to neoplasm.