Moreover, the expressions of myogenic regulatory factors, such as MyoD, myogenin, and Pax-7, were decreased (Figure 3(c)), and the expressions of catabolic factors, such as atrogin-1, MuRF-1, and myostatin, were increased in the muscles of CKD mice, relative to the Sham group (Figure 3(d)). This evidence concerns the gene MSTN and chronic kidney disease.