BIRC5 and breast carcinoma: Full length coding sequence of BIRC5 (516 bp) was amplified from a confirmed case of dog complex mammary carcinoma showing about 60 fold overexpression of BIRC5 gene (by quantitative real-time PCR analysis) as compared to normal mammary gland tissue25 and cloned in pET32b(+) prokaryotic expression vector as described in materials and methods.