SIRT1 and hepatocellular carcinoma: Additionally, experiments on tumor spheres and liver cancer samples confirm the marked downregulation of miR-486 associated with LCSC stemness features while reduced expression of miR-486 repressed the self-renewal and invasion of CSCs in HCC development via targeting sirtuin 1 (Sirt1), which plays a critical role in the maintenance of the stemness and tumorigenic potential of LCSCs [177].