As expected, overexpression of Dock1 considerably rescued the invasion that reduced by miR-486-5p, and knockout of Dock1 prominently reversed the invasion that increased by anti-miR-486-5p (Figure 4A), suggesting that the inhibiting effect of miR-486-5p on breast cancer cells is mainly carried out by downregulation of Dock1. This evidence concerns the gene DOCK1 and breast carcinoma.