And three different analyzing software including Oncomine database, PPI network and GEPIA software all predicted the positive correlation between TOP2A and TPX2, and qRT-PCR experiment conducted on 30 paired local hospital adenocarcinoma and squamous cell carcinoma samples validated the association between two genes, indicating TPX2 is a probable co-working partner of TOP2A. The gene discussed is TOP2A; the disease is squamous cell carcinoma.