TNF and colon adenocarcinoma: Similarly, previous comparative studies revealed that EBN had increased proliferation rate of the human colonic adenocarcinoma cell line (Caco-2 cells) about 135%-215% compared to the control and reduced the percentage of tumor necrosis factor-alpha in vitro [26,27] (Aswir and Wan Nazaimoon, 2011; Vimala et al., 2012).