Gene expression analysis by nanocapillary qRT-PCR (624 genes) and 1536 well high-throughput qRT-PCR (62 genes) resulted in the selection of lung cancer markers associated with higher (TMEM45A, SLC16A3, CD66, SLC2A1, CA9, CD24) or lower (EGFR) expression in vivo or in 3D models compared to monolayer cultures (Table 2, Figure 4 and Figure 5). The gene discussed is TMEM45A; the disease is lung carcinoma.