The number of technical modifications of HC reactions, evaluation of different regions of tumor (epithelium/complete mucosa), lack of unified tissue staining grading systems and difficulties in conduction of meta-analysis of changes in mucin glycoconjugates during colon carcinogenesis, motivated us to compare the results of analyses conducted with the use of two credible, fast and consistent computer-assisted techniques for HC evaluation of mucin expression in CRC. This evidence concerns the gene MUC5AC and colorectal carcinoma.