Second, we examined the anti-obesity effect of T-3601386 in a diet-induced obese (DIO) model and conducted c-Fos immunohistochemistry in the NTS after T-3601386 administration, followed by the confirmation of their GPR40 dependence using Ffar1-/- mice. This evidence concerns the gene FFAR1 and obesity due to melanocortin 4 receptor deficiency.