DM group rats showed the increased level of fibrotic parameterssuch as fibronectin (6 ± 0.42), TGF-β1(85.86 ± 3.84) and Type IV collagen (91.04 ± 4.32)and CT-PLGA-NPs significantly (p < .001) reduced the level offibronectin (3.2 ± 0.21), TGF-β1 (26.43 ± 2.04) and Type IV collagen (27 ± 1.04) at theend of the experimental study. The gene discussed is TGFB1; the disease is diabetes mellitus.