To interrogate the influence on differential gene expression profiles by SLC1A3 and ASNase, we performed transcriptome analysis in three cancer cell lines: PC3 (endogenous expression of SLC1A3), DU145 wild type (SLC1A3 negative), and DU145‐V5‐SLC1A3 (ectopic expression of SLC1A3). This evidence concerns the gene SLC1A3 and cancer.