The evidence shown here for the novel involvement of miR-125a-5p and miR-17-92 in the differentiation of human neutrophils is three-fold: a) miR-125a-5p is upregulated and miR-17-92 cluster is downregulated during neutrophil differentiation of leukemic HL-60 cells; b) ectopic expression of miR-125a-5p induces neutrophil differentiation in distinct human AML cell lines as well as in normal human CD34+-HPCs; c) ectopic expression of miR-17-92 cluster inhibits granulocytic differentiation in DMSO-treated HL-60 cells. Here, CD34 is linked to acute myeloid leukemia.