MYC and colorectal cancer: Next, we evaluated the therapeutic potential of targeting BRD4 in colorectal cancer cells, western blot indicated AZD5153 inhibited BRD4 expression and its important transcriptional regulator c-Myc (Figure 2.A), also it could suppress cell proliferation with dose dependent manner in human colorectal cancer cell lines HCT116 and Lovo (Figure 2.B), and the results of colonogenic assay also confirmed the potent anticancer effect of AZD5153 (Figure 2.C).