FGFR2 and esophageal squamous cell carcinoma: We also examined the levels of FGFR2 mRNA in a panel of ESCC cell lines by qPCR assays, and found that majority of the ESCC cell lines, except EC109 had significantly higher levels of FGFR2 mRNA than the immortalized NE3 cell line derived from normal human esophageal tissues (Fig. 1B-D).