To further investigate the effects of DGKζ inhibition in glioma cells, colony formation, flow cytometry analysis of the cell cycle, and tube formation assays were performed in vitro. Transfection of U251 and U87 MG cells with DGKζ shRNA resulted in a significant decrease in the cell numbers of each colony compared with nonsense shRNA transfection. Here, DGKZ is linked to glioma.