Western blot analysis showed that the phosphorylation levels of mTOR and AKT were lower in DGKζ-shRNA-infected cells than in control cells (Fig. 6), while there was no evident change in total AKT and mTOR, indicating that DGKζ silencing inactivated the AKT/mTOR pathway in glioma cells through a phosphorylation/dephosphorylation mechanism. The gene discussed is DGKZ; the disease is glioma.