Furthermore, the sensitivity and specificity of the abovementioned exosomal miRNAs were nearly 100% for discriminating the PDAC group from the CP and healthy groups.89 Madhaven et al. employed flow cytometry to examine selected PC-initiating cells (PaCICs) markers (CD44v6, Tspan8, EpCAM, MET, and CD104) in exosomes from patient serum and used qRT-PCR to measure miRNA levels (miR-1246, miR-4644, miR-3976, and miR-4306) in serum exosomes and exosome-depleted serum. The gene discussed is MET; the disease is pachyonychia congenita.