We also investigated the mechanism of expression of CHL1 using qPCR and western blot analysis (Fig. 1B & 1C) in 3 NPC cell lines (C666, SUNE1 and CNE2) and 2 immortalized nasopharyngeal (NP) cell lines (NP69 and NP460). This evidence concerns the gene CHL1 and nasopharyngeal carcinoma.