To determine the function of SLC39A8 mutants associated with CDG and Leigh syndrome, four mutant alleles were constructed and transfected into HeLa cells, and the results were compared with the consensus (wild-type) SLC39A8 cDNA; the selected SLC39A8 mutants included Gly38Arg, Gly38Arg + Ile340Asn, Val33Met + Gly204Cys + Ser335Thr, and Cys113Ser [19]. This evidence concerns the gene SLC39A8 and Leigh syndrome.