The second step of this approach enabled migration and unaltered proliferation of cancer cells only for several days, however, in late passages GFAP-positive cells tended to become large, non-proliferating (statistically significant for GB6 and GB9, p = 0.005 and p = 0.045, respectively) and unable to adhere to culture plate following passage (Fig. 2d-e). Here, GFAP is linked to cancer.