In addition, we measured the concentrations of plasma cytokines, including IL-35, IL-10, IL-17, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ, and further evaluated their correlations among the abundances of the Breg subsets, the concentrations of plasma cytokines, and other clinical indicators of SLE patients. The gene discussed is TNF; the disease is systemic lupus erythematosus.