The groups of in vitro assays were listed as follows: tumour-bearing macrophage alone was considered as the control group; tumour-bearing eosinophils + tumour-bearing macrophage group; cryo-thermal eosinophils + tumour-bearing macrophage group; cryo-thermal eosinophils + tumour-bearing macrophage + anti-IFN-γ antibody, termed as IFN-γ neutralizing group; cryo-thermal eosinophils + tumour-bearing macrophage + anti-IL-4 antibody, termed as IL-4 neutralizing group. The gene discussed is IL4; the disease is neoplasm.