CD4 and metastatic neoplasm: Cryo-thermal-activated eosinophils could promote M1 macrophage polarization, and DCs maturation, meanwhile, cryo-thermal-activated eosinophils could directly promote CD4+ T cells differentiation into CD4+ CTL and generation of cytotoxic CD8+ T cells shaping strong systematic anti-tumour immune response, which enhanced the therapeutic efficacy for metastatic tumour.