Meanwhile, cryo-thermal eosinophils were co-cultured with tumour-bearing DCs, adding isotype IgG1 (cryo-thermal eosinophils + tumour-bearing DCs), IFN-γ neutralizing antibody (cryo-thermal eosinophils + tumour-bearing DCs + anti IFN-γ, termed as IFN-γ neutralizing group), IL-4 neutralizing antibody (cryo-thermal eosinophils + tumour-bearing DCs + anti-IL-4 antibody, termed as IL-4 neutralizing group), respectively. Here, IL4 is linked to neoplasm.