After IFN-γ neutralizing, the gene expression of M1-associated co-stimulatory molecules and cytokines (CD86 and IL-12) was reduced and the expression of immunoregulatory molecules (CCL17, CCL22, IL-10 and iNOS) was higher than cryo-thermal eosinophils + tumour-bearing macrophage group, though the expression of M1-associated cytokines TNF-α was increased (Fig. 5E,F). Here, NOS2 is linked to neoplasm.