This confirmed that B cell depletion led to a marked reduction of both CD8+ and CD4+ T cells (one-sided Wilcoxon rank sum test, BH adjusted p < 0.01, 0.06 respectively) at the invasive tumor-stroma margin without significantly affecting total cell numbers (two-sided t-test, p = 0.9, t = −0.02, df = 52, Fig. 6a, Supplementary Fig. 8, A, B). This evidence concerns the gene CD8A and neoplasm.