To investigate how ZIKV infection influences trophoblast differentiation, qRT-PCR was used to compare the expression of TE markers CDX2, TEAD4, and ELF5, and trophoblast markers CGA, CGB, and GCM1, in TECs infected with MOCK, high (MOI = 1), or low dose (MOI = 0.001) of ZIKV. The gene discussed is ELF5; the disease is Zika virus infectious disease.